Anaerobic incubation of membrane filter cultures for improved detection of fecal coliforms from recreational waters

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Anaerobic incubation of membrane filter cultures for improved detection of fecal coliforms from recreational waters.

Anaerobic incubation of membrane filter cultures significantly enhanced detection of fecal coliforms in surface-water samples from recreational beaches. In contrast to standard aerobic incubation, anaerobic incubation suppressed overgrowth of masking, noncoliform bacteria but did not increase the frequency of fecal coliform recovery.

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A two-layer membrane filtration (MF) medium (injury-mitigating MF [IM-MF]) and a procedure for the enumeration of injured fecal coliforms are described. These procedures included the addition of glycerol and acetate plus reducing agents to both layers of a two-layer medium and rinsing of the filter with a rich resuscitation medium. Some changes in incubation time and temperatures were used. Thi...

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Membrane filter method for recovery of fecal coliforms in chlorinated sewage effluents.

The standard one-step M-FC broth-membrane-filter procedure for recovery of fecal coliforms from chlorinated sewage effluents is much less effective than the multiple-tube (most-probable-number) technique. A two-step membrane-filter method, using a pre-enrichment technique with phenol red lactose broth and incubation at 35 degrees C for 4 h, followether 18+/-2 h, enhanced fecal coliform recovery...

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Improved membrane filtration media for enumeration of total coliforms and Escherichia coli from sewage and surface waters.

Two media were developed that allowed both a total coliform count and an Escherichia coli count to be determined on the same medium after 24 h of incubation at 35 degrees C. The new media were tested along with two standard media on 10 surface water and 7 sewage samples. The experimental media yielded equivalent or higher counts relative to the standard media and recovered more specifically the...

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Elimination of overgrowth in delayed-incubation membrane filter test for total coliforms by m-ST holding medium.

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1984

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.48.2.324-326.1984